![]() Heat the samples and 95 degrees C for five to 10 minutes in a sample buffer containing a reducing agent such as beta-mercaptoethanol. The proteins are then transferred onto a membrane where they can be detected using antibodies. The purpose of western blotting is to separate proteins on a gel according to the molecular weight. ![]() Protocols are provided by Abcam “AS-IS” based on experimentation in Abcam’s labs using Abcam’s reagents and products your results from using protocols outside of these conditions may vary.
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